Titolo progetto: Ribose-seq profile and analysis of ribonucleotides in DNA of oxidatively-stressed and cancer cells

Programma di finanziamento: NIH USA

Responsabile scientifico: prof. Gianluca Tell

Collaboratori: 

Ruolo del DAME: subcontratto della Georgia Tech Research Corporation

Descrizione generale:

Studies are designed to determine for the first time the identity and distribution of ribonucleotides (rNMPs) incorporated in DNA upon oxidative stress of normal, cancer or base excision repair (BER)-defective cells by utilizing the ribose-seq technique developed in the PI’s laboratory. Oxidative stress, which is both a causative factor and a common stress in cancer cells due to dysfunctional redox regulation, can convert deoxyribonucleotides into ribonucleotides by oxidizing the deoxyribose sugar of DNA to ribose. The rNMP spectra from yeast, mouse embryonic fibroblasts, human cancer cells and cell lines, will be analyzed and compared with mutation spectra of the same cells to identify specific signatures of rNMP incorporation as biomarkers occurring in cancer cells and upon oxidative stress conditions, to reveal any link between rNMP and mutation distribution, and to provide important insights in the comprehension of cancer etiology and biology, the effect of oxidative toxicants on DNA stability and the role of BER on RNA substrates to preserve genome integrity.

Date inizio e fine progetto: 01.08.2016 – 30.04.2021

Budget totale del progetto: 326.210,00€

Sito web: sito ufficiale NIH

Contatti: Gianluca Tell